Download or read book Development of Fragmentation Techniques in Mass Spectrometry for Biological Applications written by Hong Ji and published by . This book was released on 2007 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last chapter I present our new discovery that FAB mass spectrometry in the negative ion mode can be used to unambiguously distinguish between cis- and trans- isomers of monounsaturated fatty acids by the relative strengths of two signals in the mass spectrum. I show the results of six fatty acids and demonstrate that under normal FAB ionization/desorption conditions, the deprotonated molecules [M-H]- of fatty acids undergo CRF. A characteristic fragmentation pattern of two intense clusters of mass peaks with three weak intervening peak-clusters can be used in each case to identify the position of the double bond. I also discuss the possibility of REC occurring during the FAB process.